SCR530

STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of mouse & human induced pluripotent stem cells.

• UNSPSCコード: 12352207
• eCl@ss: 32161000
• NACRES: NA.81

特性

• 品質水準: 100
• メーカー／製品名: STEMCCA
• テクニック: cell culture | stem cell: suitable
• 入力: sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)
• 輸送温度: dry ice

詳細

詳細

The STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs), respectively. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

構成

1. EF1α-STEMCCA (OKSM) Lentivirus: (Part number CS204279) Three (3) vials containing 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.

品質

Tested to confirm the generation of iPS cells from p3 mouse embryonic fibroblasts and p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable