MABT388

Anti-ADAM 17 Antibody, clone 16C11.1 | MABT388

clone 16C11.1, from mouse

• 別名: Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 16C11.1, monoclonal
• 化学種の反応性: human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_003174
• UniProtアクセッション番号: P78536
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ADAM17(6868)

詳細

詳細

Disintegrin and metalloproteinase domain-containing protein 17 (EC 3.4.24.86; UniProt P78536; also known as ADAM 17, CD156b, Snake venom-like protease. TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor necrosis factor alpha-converting enzyme) is encoded by the ADAM17 (also known as CD156B, CSVP, NISBD, NISBD1, TACE) gene (Gene ID 6868) in human. Originally described as a TNF–alpha converting enzyme, ADAM17 is known to process the cleavage of many transmembrane proteins, including membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 was the first ′sheddase′ identified. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. ADAM 17 is initially translated in its prepro-form and subsequent cleavages of the signal (aa 1-17) and propeptide (18-214) sequences produces the 610 aa mature ADAM 17 (aa 215-824).

特異性

Expected to react with both spliced isoforms.

免疫原

GST-tagged recombinant protein corresponding to human ADAM 17.

アプリケーション

Research Category
細胞骨格

Research Sub Category
接着分子（CAM）

This mouse Monoclonal Anti-ADAM 17 Antibody, clone 16C11.1, Cat. No. MABT388 detects levels of ADAM17, and has been published and validated for use in Western Blotting, Immunohistochemistry (Paraffin).

Western Blotting Analysis: 5.0 µg/mL from a representative lot detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ADAM 17 in human testis and colorectal cancer tissues.

品質

Evaluated by Western Blotting in human acute myelogenous leukemia KG-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.

ターゲットの説明

~120 kDa observed. A lower band ~100 kDa is also observed when using a higher antibody concentration (5 µg/mL). Due to glycosylation, target band size appears larger than the calculated molecular weights (93.0 kDa/prepro-isoform A, 68.2 kDa/mature isoform A, 78.5kDa/prepro-isoform B, 63.7 kDa/mature isoform B). Uncharacterized band(s) may appear in some lysates.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable