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特異性
Clone 8H6.8 is a mouse monoclonal antibody that detects biotin-conjugated proteins.
免疫原
Biotin conjugated to KLH.
アプリケーション
Quality Control Testing
Evaluated by ELISA with Biotin-BSA.
ELISA Analysis: Serial dilutions from a representative lot detected Biotin-BSA in ELISA application.
Tested Applications
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Biotin-BSA.
Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected Biotin-conjugates in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by ELISA with Biotin-BSA.
ELISA Analysis: Serial dilutions from a representative lot detected Biotin-BSA in ELISA application.
Tested Applications
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Biotin-BSA.
Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected Biotin-conjugates in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Biotin, a bicyclic compound, is a water-soluble vitamin with a ureido (N-CO-N) and a tetrahydrothiophene ring. The ureido group is involved in its binding to avidin. The tetrahydrothiophene group is a valeric acid moiety. Although biotin molecule can exist in eight stereoisomers, the D-biotin isomer is the only one with biological activity. It plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth, and development. Its deficiency, particularly during pregnancy, leads to embryonic growth retardation, congenital malformation, and death. In mammals, biotin serves as a cofactor for four carboxylases that catalyze the transfer of a carboxyl group to targeted substrates. The four biotin-dependent carboxylases are: pyruvate carboxylase; propionly-CoA carboxylase; β-methylcrotonyl-CoA carboxylase; and acetyl-CoA carboxylase. These carboxylases play a critical role in the intermediate metabolism of gluconeogenesis, fatty acid synthesis, and amino acid catabolism. It is also reported to play a role in normal immune functions, including the production of antibodies, normal macrophage function, and the differentiation of T and B lymphocytes. This monoclonal antibody recognizes biotin conjugated to various proteins in ELISA and other immunochemical applications. (Ref.: Said, SM. (2012). Subcell. Biochem. 56; 1-19; Rabin, BS. (1983). J. Nutr. 113(11); 2316 2322).
物理的形状
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
再構成
1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommended storage: +2°C to +8°C.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS2321-100UG:
MABS2321-25UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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