MABS2273

Anti-CD200/HPi1 Antibody, clone HIC0-4F9

• 別名: OX-2 membrane glycoprotein
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: HIC0-4F9, monoclonal
• 分子量: calculated mol wt 31.26 kDa; observed mol wt ~N/A kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 包装: antibody small pack of 100
• テクニック: flow cytometry: suitable; immunohistochemistry: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_001004196
• UniProtアクセッション番号: P41217
• 保管温度: 2-8°C
• 遺伝子情報: human ... CD200(4345)

詳細

特異性

Clone HIC0-4F9 is a mouse monoclonal antibody that detects CD200/HPi1 protein.

免疫原

Human pancreatic enriched islet cells.

アプリケーション

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Tested Applications

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications in fresh-frozen normal pancreas tissues (Courtesy of Craig Dorrell, Ph.D., Oregon Health & Science University, Portland, Oregon USA).

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications (Dorrell, C., et al. (2008). Stem Cell Res. 1(3):183-94).

Flow Cytometry Analysis: A representative lot detected CD200/HPi1 in Flow Cytometry applications (van der Heide, V., et al. (2022). Cell Rep. 38(11):110508).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

OX-2 membrane glycoprotein (UniProt: P41217; also known as CD200) is encoded by the CD200 (also known as MOX1, MOX2, My033) gene (Gene ID: 4345) in human. CD200 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-359), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

再構成

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable