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由来生物
mouse
品質水準
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
2D12C6, monoclonal
化学種の反応性
rat, human
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG2b
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... EPHB2(2048) , EphB2(2048)
rat ... EphB2(313633)
詳細
Ephrin type-B receptor 2 (EphB2) is a receptor tyrosine kinase for ephrin ligands that mediate numerous developmental processes, particularly in the nervous system. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB2 is critical for embryonic/postnatal development for lateral suprapyramidal blade formation which is in a specific region of the dentate gyrus. EphB2 is expressed in the two main components of the thymus, developing thymocytes, TEC, colorectal cancer, and in the earliest SGZ progenitor cells as they migrate during development and form the dentate gyrus.
免疫原
Purified recombinant fragment of EphB2 expressed in E. coli.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
ニューロン発生
ニューロン発生
Anti-EphB2 Antibody, clone 2D12C6 is a highly specific mouse monoclonal antibody, that targets EphB2 & has been tested in western blotting, IHC & ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected EphB2 in HeLa and HepG2 cells.
Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blot in IMR-32 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.
ターゲットの説明
~53 kDa observed
物理的形状
Unpurified
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
IMR-32 cell lysate
IMR-32 cell lysate
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN726:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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