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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
HTF-1, monoclonal
分子量
calculated mol wt 33 kDa
observed mol wt ~42-48 kDa
精製方法
using protein G
化学種の反応性
human, bovine
包装
antibody small pack of 100
テクニック
ELISA: suitable
dot blot: suitable
flow cytometry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
アイソタイプ
IgG1κ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
-10 to -25°C
遺伝子情報
human ... F3(2152)
特異性
Clone HTF-1 is a mouse monoclonal antibody that specifically detects Tissue Factor/CD142 protein.
免疫原
Full length native (purified) human Tissue Factor.
アプリケーション
Immunofluorescence Microscopy
Tissue factor: Identification and characterization of cell types in human placentae. (Faulk, W.P., C.A. Labarrere, and S.D. Carson. (1990). Blood 76:86-96).
Immunofluorescent studies of tissue factor on U87MG cells: Evidence for non-uniform distribution. Carson, S.D., and S.J. Pirruccello. (1993). Blood Coagulation and Fibrinolysis 4:911-920).
Flow Cytometry
Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient. (Carson, S.D., S.J. Pirruccello, and W.D. Haire. (1990). Thrombos. Res. 59:159-170).
Immunohistochemistry
Participation of cell-mediated immunity in the deposition of fibrin in glomerulonephritis. (Neale, T.J., P.G. Tipping, S.D. Carson, and S.R. Holdsworth. (1988). Lancet, No. 8606, 2:421-424).
Tissue factor antigen in senile plaques of Alzheimer′s disease. (McComb, R.D., K.A. Miller, and S.D. Carson. (1991). Am. J. Path. 139:491-494).
Immunoaffinity purification of the tissue factor protein
An inhibitory monoclonal antibody against human tissue factor. (Carson, S.D., S.E. Ross, R. Bach, and A. Guha. (1987). Blood 70:490-493).
Tissue factor: Identification and characterization of cell types in human placentae. (Faulk, W.P., C.A. Labarrere, and S.D. Carson. (1990). Blood 76:86-96).
Immunofluorescent studies of tissue factor on U87MG cells: Evidence for non-uniform distribution. Carson, S.D., and S.J. Pirruccello. (1993). Blood Coagulation and Fibrinolysis 4:911-920).
Flow Cytometry
Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient. (Carson, S.D., S.J. Pirruccello, and W.D. Haire. (1990). Thrombos. Res. 59:159-170).
Immunohistochemistry
Participation of cell-mediated immunity in the deposition of fibrin in glomerulonephritis. (Neale, T.J., P.G. Tipping, S.D. Carson, and S.R. Holdsworth. (1988). Lancet, No. 8606, 2:421-424).
Tissue factor antigen in senile plaques of Alzheimer′s disease. (McComb, R.D., K.A. Miller, and S.D. Carson. (1991). Am. J. Path. 139:491-494).
Immunoaffinity purification of the tissue factor protein
An inhibitory monoclonal antibody against human tissue factor. (Carson, S.D., S.E. Ross, R. Bach, and A. Guha. (1987). Blood 70:490-493).
Quality Control Testing
Evaluated by Western Blotting in A-431cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Tissue Factor/CD142 in A-431 cell lysate.
Tested Applications
ELISA Analysis: A representative lot detected Tissue Factor/CD142 in ELISA applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Western Blotting Analysis: A representative lot detected Tissue Factor/CD142 in Western Blotting applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3). .
Dot Blot: A representative lot detected Tissue Factor/CD142 in Dot Blot applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Inhibition Assay: A representative lot detected Tissue Factor/CD142 in Inhibition applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in A-431cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Tissue Factor/CD142 in A-431 cell lysate.
Tested Applications
ELISA Analysis: A representative lot detected Tissue Factor/CD142 in ELISA applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Western Blotting Analysis: A representative lot detected Tissue Factor/CD142 in Western Blotting applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3). .
Dot Blot: A representative lot detected Tissue Factor/CD142 in Dot Blot applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Inhibition Assay: A representative lot detected Tissue Factor/CD142 in Inhibition applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Tissue factor (UniProt: P13726; also known as TF, Coagulation factor III, Thromboplastin, CD142) is encoded by the F3 gene (Gene ID: 2152) in human. Tissue factor (TF) is an integral membrane protein that serves as a high-affinity receptor and cofactor for factor VII/VIIa. It is expressed on perivascular cells and epithelial cells at organ and body surfaces where it forms a hemostatic barrier. It is also reported to provide hemostatic protection to brain, lung, and heart. In normal healthy individuals, levels of TF are very low. Cell-surface levels of TF do not display any directly correlation with its procoagulant activity indicating that TF exists on the cell surface as dormant (encrypted) and active (de-encrypted) form. This allows for a rapid hemostatic response without a need for transcriptional upregulation of the F3 gene. TF normally exists in a partially encrypted state and its de-encryption on circulating monocytes, platelets or endothelial cells by inflammatory mediators can lead to thrombosis. It initiates blood coagulation by forming a complex with circulating factor VII or VIIa and this complex activates factor IX and X by specific, limited proteolysis. TF expression by nonvascular cells plays an essential role in homeostasis; however, TF expression by vascular cells can induce life-threatening intravascular thrombosis in various diseases, such as sepsis, cancer, and atherosclerosis. Inhibition of TF-Factor VIIa activity is shown to reduce coagulation and mortality in animal models of sepsis. Many cancer cells express large amounts of TF, which contributes to tumor angiogenesis and growth. Cancer cells can release TF-positive microparticles into the circulation that can trigger venous thromboembolism. TF on circulating tumor cells can also coat cells with fibrin that traps them within the microvasculature and promote hematogenous metastasis. (Ref.: Carson, S.D., et al. (1987). Blood. 70(2):490-3; Caldwell, JA., et al. (2010). Lab. Invest. 90; 953-962; Kasthuri, RS., et al. (2009). J. Clin. Oncol. 27(29); 4834-4838; Mackman, N. (2004).
物理的形状
Purified mouse monoclonal antibody IgG1 in PBS without azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF3203-100UG:
MABF3203-25UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
T J Neale et al.
Lancet (London, England), 2(8608), 421-424 (1988-08-20)
Fibrin deposition is prominent in delayed-type hypersensitivity (DTH) reactions and is initiated by antigen-specific, T-lymphocyte-directed macrophage expression of human tissue factor (HTF). To examine the role of DTH in glomerular fibrin deposition, 10 fibrin-positive and 24 fibrin-negative biopsy specimens from
S D Carson et al.
Thrombosis research, 59(1), 159-170 (1990-07-01)
Tissue factor activity and antigen were measured in promyelocytes freshly isolated from a patient with acute promyelocytic leukemia (APL), FAB M3. Determination of functional activity revealed that physically disrupted cells expressed considerable tissue factor of which less than two percent
R D McComb et al.
The American journal of pathology, 139(3), 491-494 (1991-09-01)
Tissue factor (tissue thromboplastin) is the primary initiator of the extrinsic coagulation pathway, triggering a proteolytic cascade when exposed to circulating coagulation factors. In this study, the distribution of tissue factor was examined immunohistochemically in Alzheimer's disease (AD) and control
S D Carson et al.
Blood, 70(2), 490-493 (1987-08-01)
We obtained a hybridoma using immune spleen cells from a mouse injected with human brain tissue factor that had been purified on a factor VII-agarose affinity column. This monoclonal IgG1, HTF1-7B8, inhibits tissue factor procoagulant activity. The concentration of HTF1-7B8
W P Faulk et al.
Blood, 76(1), 86-96 (1990-07-01)
This is an immunohistologic study of tissue factor (TF) in snap frozen, unfixed, human normal-term placentae. Antibodies to TF were a monoclonal to human brain TF purified on a factor VII-agarose affinity column, and a polyclonal to a synthetic polypeptide
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