MABE920

Anti-Mi-2β Antibody, clone 17H11

clone 17H11, from mouse, purified by affinity chromatography

• 別名: Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 17H11, monoclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• テクニック: ChIP: suitable (ChIP-seq); western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_001264
• UniProtアクセッション番号: Q14839
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CHD4(1108)

詳細

詳細

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

特異性

Recognizes Mi-2β (CHD-4)

免疫原

Recombinant protein corresponding to Human Mi-2β (CHD-4).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

ヒストン修飾タンパク質

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.

ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.

This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.

品質

Evaluated by Western Blotting in a Jurkat cell lystate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.

ターゲットの説明

~ 220 kDa observed

物理的形状

Protein G Purified

Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable