MABE1115

Anti-Pax1 Antibody, clone 5A2

from rat

• 別名: Paired box protein Pax-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: monoclonal
• 化学種の反応性: mouse, human
• 包装: antibody small pack of 25 μg
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P09084
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Pax1(18503)

詳細

詳細

Paired box protein Pax-1 (UniProt: P09084) is encoded by the Pax1 (also known as Pax-1) gene (Gene ID: 18503) in murine species. Pax-1 is a transcriptional activator that plays a role in the formation of segmented structures of the embryo and has important role in the normal development of the vertebral column. Mutations in Pax1 gene are associated with vertebral malformations. Undulated homozygous mice exhibits vertebral malformations along the entire rostro-caudal axis. Pax genes are vital for the specification of progenitor cells and for maintenance of progenitor cell fate, including that of cancer cells. Pax1 gene is silenced by methylation in ovarian and cervical cancers and may serve as a tumor suppressor gene. The methylation levels of Pax1 gene is significantly higher in tumor tissues compared to non-tumor paracancerous tissues. Hence, methylation status of Pax1 may serve as useful biomarkers for the detection of cervical cancer and is also a good prognostic indicator for oral squamous cell carcinoma. (Ref.: Huang J et al (2017). Int. J. Environ. Res. Public Health. 14(2). pii: E216).

特異性

Clone 5A2 detects Paired box protein Pax-1 in murine tissues.

免疫原

GST-tagged full-length murine recombinant paired box protein Pax-1.

アプリケーション

Research Category
アポトーシス及び癌

Detect Paired box protein Pax-1 using this rat monoclonal Anti-Pax1, clone 5A2, Cat. No. MABE1115-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A representative lot detected Pax1 in Western Blotting applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]).

Immunohistochemistry Analysis: A representative lot detected Pax1 in Immunohistochemistry applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]; Kist, R., et. al. (2014). PLoS Genet. 10(10):e1004709).

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Pax1 in Embryonic mouse head (E14.5) (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected Pax1 in d11.5 embryonic vertebral column tissue lysates (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

品質

Evaluated by Western Blotting in mouse esophagus tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Pax1 in 10 µg of m ouse esophagus tissue lysate.

物理的形状

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable