由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
2A2, monoclonal
化学種の反応性
mouse, human
テクニック
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ING3(54556)
詳細
Inhibitor of growth protein 3 (UniProt: Q9NXR8; also known as ING3, p47ING3) is encoded by the ING3 (also known as HSPC301) gene (Gene ID 54556) in human. ING3 is a ~50 kDa protein that belongs to a family of proteins that contain PHD-finger domain and act as readers of the epigenetic code through specific recognition of trimethylated lysine 4 of histone H3. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex, which is involved in transcriptional activation of select genes, principally by acetylation of nucleosomal histones H4 and H2A. This complex is also shown to activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. ING3 is highly expressed in small intestine, bone marrow, epidermis, and in tissues where cells undergo rapid proliferation and renewal and its expression is shown to be higher in proliferating cells. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. ING3 is reported to be down-regulated in squamous cell carcinoma of the head and neck.
特異性
Clone 2A2 detected recombinant human ING3, but not INGs 1, 2, 4 or 5. Target band detection by Western blotting and immunocytochemistry. Staining by clone 2A2 was abolished using ING3 shRNA-transfected cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
免疫原
GST-tagged recombinant full-length human ING3.
アプリケーション
Detect ING3 using this mouse monoclonal Anti-ING3 Antibody, clone 2A2, Cat. No. MABC1185, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ING3 immunoreactivity in human small intestine tissue section.
Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
品質
Evaluated by Immunohistochemistry in human ovarian cancer tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.
ターゲットの説明
46.74 kDa calculated. ~50 kDa reported (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
適用法令
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Jan Code
MABC1185:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Alessandra Chesi et al.
Nature communications, 10(1), 1260-1260 (2019-03-21)
Osteoporosis is a devastating disease with an essential genetic component. GWAS have discovered genetic signals robustly associated with bone mineral density (BMD), but not the precise localization of effector genes. Here, we carry out physical and direct variant to gene
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