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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
3E3, monoclonal
化学種の反応性
human
メーカー/製品名
Chemicon®
テクニック
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... FN1(2335)
特異性
The antibody reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin (Pierschbacker et al., 1981) comprimising the cell attachment site (Pierschbacker et al., 1981; Pierschabacker et al., 1983, 1984; Hayman et al., 1985; Ruoslahti et al., 1986; Hynes, 1985). It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface (Pierschbacker et al., 1981). Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser (Pierschabacker et al., 1984; Hayman et al., 1985; Ruoslahti et al., 1986).
免疫原
Epitope: cell attachment fragment
Purified human-fibronectin from amnion liquid.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
ECMタンパク質
ECMタンパク質
Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3 is an antibody against Fibronectin Frag for use in ELISA, WB, IC, IH.
Immunocytochemistry: (5-20 μg/mL) Immunohistochemistry: (5-20 μg/mL) (Not tested on paraffin sections) Immunoblotting: (5-20 μg-mL)
ELISA
Inactivation or inhibition studies in cell culture.
Optimal working dilutions must be determined by end user.
ELISA
Inactivation or inhibition studies in cell culture.
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
保管および安定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB88916:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Journal of biomedical materials research. Part A, 100(1), 18-25 (2011-10-06)
Conformational changes of fibronectin (Fn) deposited on poly(methyl methacrylate) and poly(acrylic acid) block copolymers with identical chemical compositions were detected using an antibody-functionalized atomic force microscope (AFM) tip. Based on the antibody-protein adhesive force maps and phase imaging, it was
Oncogenic targeting of BRM drives malignancy through C/EBP?-dependent induction of ?5 integrin.
Oncogene null
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Cells respond to extracellular mechanical forces through the assembly of integrin adhesion complexes (IACs) that provide a scaffold through which cells sense and transduce responses to those forces. IACs are composed of transmembrane integrin receptors that bind to extracellular matrix
Developmental dynamics : an official publication of the American Association of Anatomists, 238(5), 1118-1130 (2009-04-23)
In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for
Cell and tissue research, 360(2), 245-262 (2015-02-11)
Cochlear micromechanics and frequency tuning depend on the macromolecular organization of the basilar membrane (BM), which is still unclear in man. Novel techniques in cochlear implantation (CI) motivate further analyses of the BM. Normal cochleae from patients undergoing removal of
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