MAB16985
Anti-MCAM Antibody, clone P1H12
clone P1H12, Chemicon®, from mouse
別名:
CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
P1H12, monoclonal
化学種の反応性
mouse, canine, human
以下との反応禁止
rat
包装
antibody small pack of 25 μg
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG1
適合性
not suitable for immunohistochemistry (Paraffin)
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MCAM(4162)
関連するカテゴリー
詳細
MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
特異性
Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
免疫原
Immunization of mice with HUVEC.
アプリケーション
Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
Immunocytochemistry:
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
1-10 μg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.
Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or <2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.
Immunoprecipitation:
1-10 μg/mL of a previous lot worked in immunoprecipitation.
ELISA:
1-10 μg/mL of a previous lot worked in ELISA.
FACS Analysis:
1-10 μg/mL of a previous lot worked in FACS.
Optimal working dilutions must be determined by end user.
品質
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
ターゲットの説明
110 kDa
物理的形状
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.
アナリシスノート
Control
HUVEC cells.
HUVEC cells.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB16985-25UG:
MAB16985:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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