EZRGRT

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies total Ghrelin in 20 μL serum or plasma

• 別名: Appetite Regulating Hormone, Growth hormone-releasing peptide, Interleukin-6, Motilin-related peptide
• UNSPSCコード: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

特性

• product name: Rat/Mouse Ghrelin (total) ELISA, This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.
• 品質水準: 200
• 化学種の反応性: mouse, rat
• 包装: kit of 1 × 96 wells
• パラメーター: 20 μL sample volume (4hr assay)
• assay range: accuracy: 80-103%; (Serum: Active Ghrelin); accuracy: 86-102%; (Plasma: Active Ghrelin); accuracy: 92-103%; (Serum: Des-octanoyl Ghrelin); accuracy: 95-107%; (Plasma: Des-octanoyl Ghrelin); inter-assay cv: 1.8-4.5%; intra-assay cv: 0.7-1.3%; (Serum); inter-assay cv: 2.0-3.2%; intra-assay cv: 1.1-1.7%; (Plasma)
• テクニック: ELISA: suitable
• 入力: sample type plasma (K2 EDTA); sample type serum
• UniProtアクセッション番号: Q9EQX0 (mouse); Q9QYH7 (rat); Q9UBU3
• アプリケーション: research use
• 検出方法: colorimetric (450nm/590nm)
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

特異性

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

アプリケーション

Research Category
代謝

Research Sub Category
肥満

代謝障害

Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)

Used to detect/quantify: Ghrelin (Total)

その他情報

Please contact Technical Service for linearity of dilution.

免責事項

For research use only. Not for use in diagnostic procedures.

安全性情報

• ピクトグラム: GHS06,GHS05,GHS09
• シグナルワード: Danger
• 危険有害性情報: H290,H302 + H332,H311,H315,H317,H319,H335,H411
• 注意書き: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects