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Merck

ELI-008-M

Sigma-Aldrich

IL-6 ELISPOT Antibody Pair, Mouse

from rat, clone MP5-20F3, clone MP5-32C11

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rat

品質水準

クローン

MP5-20F3, monoclonal
MP5-32C11, monoclonal

化学種の反応性

mouse

テクニック

ELISA: suitable

輸送温度

wet ice

詳細

Interleukin 6 (IL-6) is a multifunctional cytokine that plays an important role in regulating immune and inflammatory responses. Produced by a wide variety of cells, including T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 works by stimulating B-cell differentiation and antibody production, as well inducing expression of hepatic acute-phase proteins, regulating bone metabolism, and promoting neuronal differentiation. In addition, IL-6 appears to interact with IL-2 in the proliferation of T-lymphocytes and with IL-3 to promote the proliferation of multipotent hematopoietic progenitor cells. The Millipore mouse IL-6 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-6 secreting cells at the single cell level.

特異性

Both the capture and the detection antibodies included in this kit react with mouse interleukin -6 (IL-6).

アプリケーション

Research Category
炎症及び免疫
IL-6 ELISPOT Antibody Pair, Mouse is a highly specific rat monoclonal antibody & that targets IL-6 & has been tested in ELISA.

包装

Sufficient reagents for five, 96-well plates.

構成

• Mouse IL-6 Capture Antibody, clone MP5-20F3. Quantity: 500 µg.

• Mouse IL-6 Detection Antibody, clone MP5-32C11. Quantity: 100 µg.

• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.

ターゲットの説明

~25 kDa

物理的形状

Format: Purified
The 500 µg purified IL-6 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and the 100 µg purified IL-6 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.

保管および安定性

Maintain refrigerated at 2-8°C for up to six months from the date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ELI-008-M:


試験成績書(COA)

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資料

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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