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Merck
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主要文書

安全性情報

CP28

Sigma-Aldrich

Anti-Fibroblast Antigen Mouse mAb (AS02)

liquid, clone AS02, Calbiochem®

別名:

Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

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About This Item

UNSPSCコード:
12352203

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

AS02, monoclonal

形状

liquid

含みます

≤0.1% sodium azide as preservative

化学種の反応性

human, monkey

以下との反応禁止

rat, mouse

メーカー/製品名

Calbiochem®

保管条件

OK to freeze

アイソタイプ

IgG1

輸送温度

wet ice

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... FGF22(27006)

詳細

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.

免疫原

Human
human fibroblasts

アプリケーション

Flow Cytometry (see comments)

Frozen Sections (10 µg/ml, acetone fixation)

Immunoblotting (10 µg/ml, non-reducing only)

Immunofluorescence (10 µg/ml)

Immunoprecipitation (2-10 µg/ml)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

物理的形状

In 50 mM sodium phosphate buffer, 6% BSA.

アナリシスノート

Positive Control
Any fibroblast cell line or skin tissue

その他情報

Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.

法的情報

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

CP28-MG:
CP28-200UG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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