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形状
liquid
包装
pkg of 2 X 175 μg
テクニック
cell culture | stem cell: suitable
保管温度
2-8°C
詳細
Cardiomyocytes are the cardiac muscle cells that make up the heart and are responsible for generating contractile forces within the heart. Laminin-221 is known to bind to the integrin α7X2β1 which is located on the cell surface of pluripotent stem cells and is known to induce cardiac and skeletal muscle differentaition. Professor Kiyotoshi Sekiguchi′s group (Matrixome, Inc.) have produced a recombinant E8 fragment of laminin-221 at large-scale while retaining the full integrin binding activity. ECMatrix-221 E8 Laminin Substrates are recombinant Laminin-221-E8 fragments which bind to integrin α7X2β1 and increases the differentiation of pluripotent stem cells (ES/iPSCs) into functional cardiomyocytes.
アプリケーション
Xeno-free laminin-221 coating for the differentiation of pluripotent stem cells into cardiomyocytes, 350 μg (CHO-S derived)
包装
350 μg (2 x175 μg)
構成
2 X 175 μg ECMatrix-221 E8 Laminin Substrate (0.5 mg/mL in PBS). Expressed in CHO-S cells.
品質
- Purity (SDS-Page): > 95%
- Endotoxin Test: = 750 EU/mg
- Mycoplasma Test: Negative
- Sterility Test: Negative
- Integrin Binding Assay (kDa): = 10 nM
調製ノート
Precoating Method
1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 μg/mL working solution.
2. Coat dishes with ECMatrix-221 at 0.25 μg/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 μg/mL working solution).
3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
4. Before use, remove remaining fluid from the coated surface (do not rinse).
5. Detach cells into small clumps using Accutase.
6. Plate the cells at desired density.
Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.
1. Dilute the 0.5 mg/mL stock solution with sterile PBS to achieve a 2.5 μg/mL working solution.
2. Coat dishes with ECMatrix-221 at 0.25 μg/cm2 (for example, for one well of a 6-well plate add 1 mL of the 2.5 μg/mL working solution).
3. Incubate for 1 hour at 37°C, 3 hours at room temperate or overnight at 4°C.
4. Before use, remove remaining fluid from the coated surface (do not rinse).
5. Detach cells into small clumps using Accutase.
6. Plate the cells at desired density.
Note: Do not allow the plates to dry, briefly spin down all liquids in the tube before use, avoid repeated freeze-thaw cycles.
保管および安定性
ECMatrix-221 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CC163-350UG:
試験成績書（COA）
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