ABT283

Anti-Cadherin-11/OB Cadherin Antibody

from rabbit, purified by affinity chromatography

• 別名: Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_001788
• UniProtアクセッション番号: P55287
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDH11(1009)

詳細

詳細

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

免疫原

Epitope: Cadherin 5 domain

KLH-conjugated linear peptide corresponding to the Cadherin 5 domain of Human Cadherin-11/OB Cadherin.

アプリケーション

Research Category
細胞骨格

Research Sub Category
接着分子（CAM）

This Anti-Cadherin-11/OB Cadherin Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Cadherin-11/OB Cadherin.

Western Blotting Analysis: 2 µg/mL of a representative lot of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Mouse Brain, Mouse Brain Cytosol, Mouse Brain Membrane, Mouse Brain Nuclear Extract, Mouse (Aged) Brain Frontal Cortex, and Mouse Cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot of this antibody detected Cadherin-11/OB Cadherin in neurons of the human cerebral cortex and thalamus, as well as purkinje and basket cells in human cerebellum.

品質

Evaluated by Western Blotting in Human Thalamus Tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Human Thalamus Tissue lysate.

ターゲットの説明

~75 kDa observed.
Full length protein has a molecular weight of ~120 kDa, however the cleaved product has a molecular weight of ~75-80 kDa (McCusker, C., et al. (2009) MBC. 20(1):78-89). Uncharacterized bands may be observed in some lysates.

物理的形状

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable