ABE991

Anti-NeuroD1 Antibody

serum, from rabbit

• 別名: Neurogenic differentiation factor 1, NeuroD1, Beta-cell E-box transcriptional activator 2, Beta2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse
• テクニック: ChIP: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NP_035024
• UniProtアクセッション番号: Q60867
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Neurod1(18012) ; rat ... Neurod1(29458)

詳細

詳細

NeuroD1 is a transcriptional activator that associates with p300 transcription co-activator protein to promote the expression of many important differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. NeuroD1 is required for amacrine cell fate as well as dendrite morphogenesis and the maintenance of cerebellar cortex. Also via its associate with chromatin, NeuroD1 helps regulate the expression of many other key transcriptional regulators of neurogenesis as well. NeuroD1 is expressed in developing brain and pancreas as well as in differentiating neurons in both the CNS and PNS systems. Disruption of NeuroD1 leads to early death in mice with several developmental and functional defects throughout the body pointing to this factors key role in development in many systems.

免疫原

GST-tagged recombinant protein corresponding to mouse NeuroD1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

Immunohistochemistry Analysis: A representative lot detected NeuroD1 in developing pituitary tissue (Lavoie, P., et al. (2008). Molecular Endocrinology. 22:1647-1657).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated NeuroD1 in AtT-20 cell lysate (Langlais, D., et al. (2011). Molecular Endocrinology. 25:348-359).

This Anti-NeuroD1 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry for the detection of NeuroD1.

品質

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NeuroD1 in 10 µg of rat brain tissue lysate.

ターゲットの説明

~40 kDa observed. Uncharacterized band(s) may appear in some lysates.

物理的形状

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1