由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, human, rat
テクニック
ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MAFK(7975)
詳細
Transcription factor MafK (UniProt Q61827; also known as Erythroid transcription factor NF-E2 p18 subunit, Nuclear factor erythroid derived 2 ubiquitous, p18 NF-E2) is encoded by the Mafk (also known as Nfe2u, AW061068) gene (Gene ID 17135) in murine species. MafK, MafG and MafF consistute a family of transcription factors that dimerize with the CNC (cap′ n′ collar) family regulatory protein Nrf2 (NF-E2 p45-related factor 2) via their bZip domain. The Nrf2/small Maf heterodimer regulates downstream genes, including those of phase II detoxifying enzymes, by targeting antioxidant responsive element (ARE) and Maf recognition element (MARE).
免疫原
Maltose binding protein-tagged recombinant protein corresponding to mouse MafK/Nfe2u.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
核受容体
核受容体
This Anti-MafK/Nfe2u Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of MafK/Nfe2u.
Western Blotting Analysis: A representative lot detected endogenous MafK as well as Zn+2-induced expression of exogenous MafK using nuclear extracts from a mouse erythroleukemia (MEL) cell line transfected with MafK cDNA in a metallothionein IIA gene promoter-driven expression plasmid (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
Chromatin Immunoprecipitation Analysis: A representative lot detected MafK association with the GST-P enhancer (GPE1) in rat hepatoma cells (H4IIE and dRLh84) and hyperplastic nodules/HN-bearing pre-neoplastic rat hepatocytes, but not in normal rat hepatocytes. While MafK association with the NAD(P)H:quinone oxidoreductase 1 (NQO1) antioxidant responsive element (ARE) was detected in all four sample types (Ikeda, H., et al. (2004). Biochem J. 380(Pt 2):515-521).
Electrophoretic Mobility Shift Assay Analysis: A representative lot caused a supershift of MafK-containing complex in EMSA using radiolabeled oligonucleotides containing PBGD gene NF-E2 binding site and nuclear extracts from a mouse erythroleukemia (MEL) cell line induced to overexpress MafK (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of both the constitutive and tBHQ-induced ARE-binding complex (Complex I and II, respectively) in EMSA using a radiolabeled oligonucleotide containing antioxidant responsive element (ARE) NF-E2 binding site and nuclear extracts from untreated and tBHQ-treated K562 cells (Kim, Y.C., et al. (2003). Oncogene. 22(12):1860-1865).
Chromatin Immunoprecipitation Analysis: A representative lot detected MafK association with the GST-P enhancer (GPE1) in rat hepatoma cells (H4IIE and dRLh84) and hyperplastic nodules/HN-bearing pre-neoplastic rat hepatocytes, but not in normal rat hepatocytes. While MafK association with the NAD(P)H:quinone oxidoreductase 1 (NQO1) antioxidant responsive element (ARE) was detected in all four sample types (Ikeda, H., et al. (2004). Biochem J. 380(Pt 2):515-521).
Electrophoretic Mobility Shift Assay Analysis: A representative lot caused a supershift of MafK-containing complex in EMSA using radiolabeled oligonucleotides containing PBGD gene NF-E2 binding site and nuclear extracts from a mouse erythroleukemia (MEL) cell line induced to overexpress MafK (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of both the constitutive and tBHQ-induced ARE-binding complex (Complex I and II, respectively) in EMSA using a radiolabeled oligonucleotide containing antioxidant responsive element (ARE) NF-E2 binding site and nuclear extracts from untreated and tBHQ-treated K562 cells (Kim, Y.C., et al. (2003). Oncogene. 22(12):1860-1865).
品質
Evaluated by Western Blotting in MEL (mouse erythroleukemia) cell lysate.
Western Blotting Analysis: 1:2,000 dilution of this antibody detected MafK/NF-E2 in 20 µg of MEL (mouse erythroleukemia) cell lysate.
Western Blotting Analysis: 1:2,000 dilution of this antibody detected MafK/NF-E2 in 20 µg of MEL (mouse erythroleukemia) cell lysate.
ターゲットの説明
~18 kDa observed. Uncharacterized band(s) may appear in some lysates.
物理的形状
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE1928:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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