ABE1859

Anti-KAP-1/TRIM28 Antibody

serum, from rabbit

• 別名: Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, KAP-1/TRI
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_005753
• UniProtアクセッション番号: Q13263
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Trim28(21849)

詳細

詳細

Transcription intermediary factor 1-beta (UniProt Q13263; also known as E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, Protein phosphatase 1 regulatory subunit 157, RING finger protein 96, TIF1-beta, Transcriptional intermediary factor 1-beta, Tripartite motif-containing 28, Tripartite motif-containing protein 28) is encoded by the TRIM28 (also known as KAP1, PPP1R157, RNF96, TF1B, TIF1B) gene (Gene ID 10155) in human.
Kruppel-associated box-/KRAB-associated protein 1 (KAP-1) is a transcriptional co-repressor that selectively targets the KRAB domain of zinc finger protein-based transcription factors with its N-terminal tripartite RBCC (RING, b-box, coiled-coil) domain. KAP-1 uses its C-terminal PxVxL motif and the PHD-Bromo domain to recruit chromatin modifiers, such as heterochromatin protein 1 (HP-1), Histone-lysine N-methyltransferase SETDB1, nucleosome remodeling and deacetylation (NuRD) complex, and histone deacetylases (HDACs), for transcriptional repression. KAP-1 also plays an essential role in the DNA damage response (DDR). Upon DNA damage, KAP-1 is rapidly phosphorylated by Ataxia telangiectasia mutated (ATM) on Ser824, causing chromatin relaxation to facilitate DNA repair. In addition, SIRT1-mediated KAP-1 deacetylation is reported to stabilize KAP1 and 53BP1 interaction, resulting in an enhancement of NHEJ-mediated repair efficiency in a KAP-1 pSer824-independent manner.

特異性

Expected to react with both spliced isoforms.

免疫原

Epitope: Bromo domain

GST-tagged recombinant protein corresponding to the Bromo domain of human KAP-1/TRIM28.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

Anti-KAP-1/TRIM28 Antibody is an antibody against KAP-1/TRIM28 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

Western Blotting Analysis: A representative lot detected the endogenous KAP-1/Trim28 only in the immunoprecipitats of exogenously expressed FLAG-SirT1 constructs that contain the KAP-1/Trim28-binding domain (a.a. 143-213) using various transfected HEK293T cells (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected similar level of endogenous KAP-1/Trim28 in HEK293T cells regardless of ionizing radiation treatment or shRNA-mediated SirT1 downregulation (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: A representative lot detected KAP-1/Trim28 in whole cell lysates, but not in BAP1, FoxK1, or FoxK2 immunoprecipitate (Okino, Y., et al. (2015). J. Biol. Chem. 16;290(3):1580-1591).
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated KAP-1/Trim28 from HEK293T cell lysates (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunocytochemistry Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 nuclear localization in U2OS cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SirT1 with KAP-1/Trim28 from HEK293T cell lysates (Lin, Y.H., et al. (2015). PLoS One. 10(4):e0123935).
Western Blotting Analysis: An 1:1000 dilution from a representative lot detected KAP-1/Trim28 in mouse embryonic fibroblast (MEF) lysate, as well as downregulated KAP-1/Trim28 in lysates from KAP1 siRNA-treated HEK293T cells (Courtesy of Dr. Zhenkun Lou, Mayo clinic, Rochester, MN).

品質

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected KAP-1/TRIM28 in 10 µg of HEK293T cell lysate.

ターゲットの説明

~110 kDa observed

物理的形状

Unpurified

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable