ABC927

Anti-LDHB Antibody

from rabbit, purified by affinity chromatography

• 別名: L-lactate dehydrogenase B chain (EC:1.1.1.27), LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), canine (based on 100% sequence homology), mouse (based on 100% sequence homology), rabbit (based on 100% sequence homology), bovine (based on 100% sequence homology), pig (based on 100% sequence homology), horse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_002291
• UniProtアクセッション番号: P07195
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LDHB(3945)

詳細

詳細

L-lactate dehydrogenase B chain (EC 1.1.1.27; UniProt P07195; also known as Epididymis secretory protein Li 281, Lactate dehydrogenase H chain, LDH-B, LDH-H, LDH heart subunit, Renal carcinoma antigen NY-REN-46) is encoded by the LDHB (also known as LDHBD, TRG-5, HEL-S-281) gene (Gene ID 3945) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. Lactate dehydrogenase (LDH) is a tetrameric enzyme composed of two different subunits, LDHA and LDHB, which can assemble into five different combinations (LDH1 is comprised of four LDHB subunits; LDH2 contains three LDHB and one LDHA subunit; LDH3 contains two LDHB and two LDHA subunits; LDH4 contains one LDHB and three LDHA subunits; LDH5 contains four LDHA subunits). LDHB is ubiquitously expressed and the predominant isoform in heart muscle, while LDHA is the predominant isoform in skeletal muscle and other highly glycolytic tissues. LDH catalyzes the reversible conversion of pyruvate into lactate. An upregulation of LDH therefore reduces the oxygen dependency and ensures an efficient anaerobic/glycolytic metabolism in tumor cells. Serum LDHB levels, for example, are reported to elevate in lung cancer patients and progressively increase with the clinical stage of non-small cell lung cancer (NSCLC).

免疫原

KLH-conjugated linear peptide corresponding to human LDHB sequence from the C-terminal half.

アプリケーション

Detect LDHb using this rabbit polyclonal Anti-LDHB Antibody, Cat. No. ABC927, validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected LDHB in normal human prostate tissue, as well as human cancer cells in Non-Hodgkin lymphoma, kidney, colon, and lung tumor tissues.

品質

Evaluated by Western Blotting in human PANC-1 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected LDHB in 10 µg of human PANC-1 cell lysate.

ターゲットの説明

~37 kDa observed

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable