ABC1414

Anti-phospho IGPR-1 (Ser220)

• 別名: Transmembrane and immunoglobulin domain-containing protein 2;CD28 homolog;Immunoglobulin and proline-rich receptor 1
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• 分子量: calculated mol wt 30.68 kDa; observed mol wt ~45 to 55 kDa
• 精製方法: using Protein A
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: monkey
• 包装: antibody small pack of 100 μg
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal half
• タンパク質IDアクセッション番号: NP_001162597.1
• UniProtアクセッション番号: Q96BF3
• 保管温度: -10 to -25°C
• ターゲットの翻訳後修飾: phosphorylation (pSer220)
• 遺伝子情報: human ... TMIGD2(126259)

詳細

詳細

Transmembrane and immunoglobulin domain-containing protein 2 (UniProt: Q96BF3; also known as CD28 homolog, Immunoglobulin and proline-rich receptor 1, IGPR-1) is encoded by the TMIGD2 (also known as CD28H, IGPR1, UNQ3059/PRO9879) gene (Gene ID: 126259) in human. IGPR-1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-282). It is widely expressed with higher expression observed in epithelial and endothelial cells, including bronchial epithelial cells of lung, breast glandular and lobular epithelia cells. It plays a role in cell-cell interaction, cell migration, and angiogenesis. IGPR-1 is localized to endothelial adherens junctions, and through trans-homophilic dimerization it regulates endothelial cell-cell adhesion and barrier function. This dimerization stimulates phosphorylation of serine 220, which is required for IGPR-1 to regulate endothelial barrier function and angiogenesis. Through interaction with HHLA2, IGPR-1 is reported to co-stimulate T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an Akt-dependent signaling cascade. IGPR-1 is also phosphorylated on serine 220 by I B kinase β, which leads to its activation. Activated IGPR-1 then stimulates phosphorylation of AMPK, which in turn phosphorylates ULK1 and Beclin-1 and leads to increased levels of LC3-II. Genotoxic agents such as doxorubicin and flow shear stress are also reported to simulate phosphorylation of IGPR-1 at serine 220. (Ref.: Amraei, R., et al. (2020). J. Biol. Chem. 295(49); 16691-16699; Wang, YHW., et al.(2016). J. Mol. Biol. 428(24; PtB); 5019-5033).

特異性

This rabbit polyclonal antibody detects Immunoglobulin and proline-rich receptor 1 (IGPR-1) phosphorylated on serine 220.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids surrounding phosphoserine 220 in the cytoplasmic domain in Human Immunoglobulin and proline-rich receptor 1 (IGPR-1).

アプリケーション

Qualtiy Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells overexpressing IGPR-1.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected IGPR-1 phosphorylated on serine 220 in lysates from HEK293 cells overexpressing IGPR-1.

Tested applications

Immunofluorescence Analysis: A representative lot detected phospho-IGPR-1 (Ser220) in Immunofluorescence applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Immunohistochemistry Applications: A representative lot detected phospho-IGPR-1 (Ser220) in Immunohistochemistry applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

物理的形状

Purified rabbit polyclonal antibody in PBS without azide.

再構成

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable