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![Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor™ 488 conjugate clone 7F9.2, from mouse, ALEXA FLUOR™ 488](/deepweb/assets/sigmaaldrich/product/images/405/633/324511fc-ecc5-4c49-9d9d-8cf25ab28d40/640/324511fc-ecc5-4c49-9d9d-8cf25ab28d40.jpg)
由来生物
rabbit
品質水準
抗体製品の状態
affinity purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
mouse, human
メーカー／製品名
Chemicon®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
入力
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... POU5F1(5460)
詳細
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
特異性
Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
免疫原
Synthetic peptide, amino acids 282-297of Oct4.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
Research Sub Category
多能性及び初期分化
多能性及び初期分化
This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
ターゲットの説明
44 kDa
物理的形状
ImmunoAffinity Purified
Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
保管および安定性
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
アナリシスノート
Control
Pluripotent human embryonic stem (ES) cells
Pluripotent human embryonic stem (ES) cells
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB3209:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Method of isolation and characterization of Girardia tigrina stem cells.
Lopes, KA; DE Campos Velho, NM; Pacheco-Soares, C
Biomedical Reports null
Nicole Renee Lee Sparks et al.
Stem cells (Dayton, Ohio), 36(3), 349-362 (2017-12-02)
Human pluripotent stem cell-derived osteoblasts possess great potential for use in bone disorder elucidation and repair; however, while the general ability of human pluripotent stem cells to differentiate into osteoblasts and lay down bone-specific matrix has been shown, previous studies
Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafish.
Poonam Sharma et al.
Life science alliance, 2(5) (2019-10-09)
Octamer-binding transcription factor 4 (Oct4, also known as Pou5F3) is an essential pluripotency-inducing factor, governing a plethora of biological functions during cellular reprogramming. Retina regeneration in zebrafish involves reprogramming of Müller glia (MG) into a proliferating population of progenitors (MGPCs)
Chawalit Siriboon et al.
PloS one, 10(2), e0118165-e0118165 (2015-02-14)
We attempted to isolate ES cell lines using inner cell masses from high-quality cloned porcine blastocysts. After being seeded onto feeders, embryos had better (P < 0.05) attachment, outgrowth formation and primary colonization in both 2× and 3× aggregated cloned
Max Villa et al.
Biomedical microdevices, 12(2), 253-261 (2009-12-17)
We present optimal perfusion conditions for the growth of primary mouse embryonic fibroblasts (mEFs) and mouse embryonic stem cells (mESCs) using a microfluidic perfusion culture system. In an effort to balance nutrient renewal while ensuring the presence of cell secreted
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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