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由来生物
sheep
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
rat, guinea pig, rabbit
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
rabbit ... Chat(100342226)
rat ... Chat(290567)
特異性
Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.
免疫原
A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経伝達物質及び受容体
神経細胞及びグリアマーカー
神経伝達物質及び受容体
神経細胞及びグリアマーカー
Immunohistochemistry: 1:1,000-1:2,000
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.
物理的形状
Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
保管および安定性
Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
E E Hur et al.
Neuroscience, 164(4), 1721-1731 (2009-09-26)
The basal forebrain (BF) comprises morphologically and functionally heterogeneous cell populations, including cholinergic and non-cholinergic corticopetal neurons that are implicated in sleep-wake modulation, learning, memory and attention. Several studies suggest that glutamate may be among inputs affecting cholinergic corticopetal neurons
Guy R Sander et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(7), 969-972 (2003-06-18)
The Notch signaling pathway is a vitally important pathway in regulating brain development. To explore the involvement of the Notch pathway in neuronal cells of adult rat gut, we investigated the expression of Notch1 and Jagged2 by in situ hybridization
S D Motts et al.
Neuroscience, 154(1), 186-195 (2008-01-29)
We used an antibody to choline acetyltransferase (ChAT) to label cholinergic cells in guinea pig brainstem. ChAT-immunoreactive (IR) cells comprise several prominent groups, including the pedunculopontine tegmental nucleus, laterodorsal tegmental nucleus, and parabigeminal nucleus, as well as the cranial nerve
Neural pathways underlying lactate-induced panic.
Johnson, PL; Truitt, WA; Fitz, SD; Lowry, CA; Shekhar, A
Neuropsychopharmacology null
Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.
Jiang, YH; Jiang, P; Yang, JL; Ma, DF; Lin, HQ; Su, WG; Wang, Z; Li, X
Testing null
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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