AB10518

Anti-DARPP-32 Antibody

from rabbit, purified by affinity chromatography

• 別名: protein phosphatase 1, regulatory (inhibitor) subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32, dopamine and cAMP regulated phosphoprotein, protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotei
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat, mouse, rhesus macaque
• 化学種の反応性（ホモロジーによる予測）: canine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_659077
• UniProtアクセッション番号: Q60829
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Ppp1R1B(19049)

詳細

詳細

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family. DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation. It possesses at least four sites that can be phosphorylated. Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor. However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation. This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors. Studies suggest that it may also have a role in the Circadian System photic pathway. Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.

特異性

This antibody recognizes DARPP-32 near the N-terminus.

免疫原

KLH-conjugated linear peptide corresponding to mouse DARPP-32 near the N-terminus.

アプリケーション

Research Sub Category
神経伝達物質及び受容体

イオンチャネル及びトランスポーター

Anti-DARPP-32 Antibody is an antibody against DARPP-32 for use in WB & IP.

Western Blot (Snap i.d.) Analysis: 1 µg/mL of this antibody from a previous lot detected DARPP-32 on 10 µg of rat brain tissue lysate.

Immunoprecipitaion Analysis: 10 µg of this antibody from a previous lot immunoprecipitated DARPP-32 from rat brain RIPA lysate.

品質

Evaluated by Western Blot on rat brain tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected DARPP-32 on 10 µg of rat brain tissue lysate.

ターゲットの説明

~ 32 kDa observed

関連事項

Replaces: AB1656

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable