71340-M

pCDFDuet-1 DNA - Novagen

• 別名: Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• UNSPSCコード: 41106602
• NACRES: NA.51

特性

• 形状: liquid
• 品質水準: 100
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze
• プロモーター: Promoter name: T7
• selection: spectinomycin
• 保管温度: −70°C

詳細

詳細

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

アプリケーション

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

警告

Toxicity: Standard Handling (A)

法的情報

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable