676602

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

lyophilized, clone 16C2, Calbiochem^®

• 別名: Anti-Vasodilator-stimulated Phosphoprotein, Activated
• UNSPSCコード: 12352203

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 16C2, monoclonal
• 形状: lyophilized
• 含みます: ≤0.1% sodium azide as preservative
• 化学種の反応性: mouse, human
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• アイソタイプ: IgG1
• 輸送温度: ambient
• 保管温度: −20°C
• ターゲットの翻訳後修飾: phosphorylation (pSer239/pSer239)

詳細

詳細

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

免疫原

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH

アプリケーション

ELISA (0.05 µg/ml)

Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 2X PBS, PEG, sucrose.

再構成

Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

アナリシスノート

Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets

その他情報

Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.

Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable