567750

Splitomicin

A cell-permeable lactone derived from β-naphthol that acts as a selective inhibitor of NAD⁺-dependent histone deacetylase activity of Sir2 protein (IC₅₀ = 60 µM).

• 別名: Splitomicin, 1,2-Dihydro-3H-naphtho[2,1-b]pyran-3-one
• 実験式（ヒル表記法）: C₁₃H₁₀O₂
• CAS番号: 5690-03-9
• 分子量: 198.22
• MDL番号: MFCD08705254
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: powder
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: off-white
• 溶解性: DMSO: 100 mg/mL
• 輸送温度: ambient
• 保管温度: 2-8°C
• InChI: 1S/C13H10O2/c14-13-8-6-11-10-4-2-1-3-9(10)5-7-12(11)15-13/h1-5,7H,6,8H2
• InChI Key: ISFPDBUKMJDAJH-UHFFFAOYSA-N

詳細

詳細

A cell-permeable and selective inhibitor of NAD⁺-dependent deacetylase activity of Sir2 protein (IC₅₀ = 60 µM). Creates a phenocopy of the sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Reported to act by either altering or blocking access to the acetylated histone-binding pocket.

A cell-permeable lactone derived from β-naphthol that acts as a selective inhibitor of NAD⁺-dependent histone deacetylase activity of Sir2 protein (IC₅₀ = 60 µM). It creates a conditional phenocopy of a sir2 deletion mutant in S. cerevisiae and sensitizes mammalian cells to a variety of DNA-damaging agents by abrogating Sir2p activity on p53. Acts by either altering or blocking access to the acetylated histone binding pocket.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
NAD⁺-dependent histone deacetylase activity of Sir2 protein

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 60 µM against NAD+-dependent histone deacetylase activity of Sir2 protein

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Bedalov, A., et al. 2001. Proc. Natl. Acad. Sci. USA98, 15113.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable