444248

MMP-13, Proenzyme, His•Tag^®, Human, Recombinant, S. frugiperda

• 別名: Collagenase-3
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• アッセイ: ≥90% (SDS-PAGE)
• 品質水準: 100
• 形状: liquid
• 比活性: ≥50 mU/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).

Note: 1 mU = 1 milliunit.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

物理的形状

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Herget, J., et al. 2003. Am. J. Physiol.285, 4199.
Nagase, H. 1997. Biol. Chem.378, 151.
Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Knauper, V., et al. 1996. J. Biol. Chem.271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.
Knight, C.G., et al. 1992. FEBS Lett.296, 263.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable