324699

Elastase Substrate IV, Colorimetric

A specific substrate for pancreatic elastase.

• 別名: Elastase Substrate IV, Colorimetric
• 実験式（ヒル表記法）: C₂₅H₃₄N₆O₉
• 分子量: 562.57
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; desiccated
• 色: white to off-white
• 溶解性: ethanol: 1 mg/mL; water: soluble
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
A specific substrate for pancreatic elastase

Product does not compete with ATP.

Reversible: no

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

警告

Toxicity: Standard Handling (A)

シーケンス

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

物理的形状

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

再構成

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

その他情報

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable