239807

Cyclopamine-KAAD

≥70% (sum of two isomers, HPLC), liquid, Hedgehog signaling inhibitor, Calbiochem^®

• 別名: 3- Keto-N-( aminoethyl- aminocaproyl- dihydrocinnamoyl)cyclopamine, 3-Keto-N-(aminoethyl-aminocaproyl-dihydrocinnamoyl)cyclopamine, InSolution Cyclopamine-KAAD
• UNSPSCコード: 12352116
• NACRES: NA.77

特性

• 製品名: Cyclopamine-KAAD, InSolution, ≥70%, inhibits Hedgehog (Hh) signaling
• 品質水準: 100
• アッセイ: ≥70% (sum of two isomers, HPLC)
• フォーム: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

A cell-permeable potent analog of Cyclopamine (Cat. No. 239803) that specifically inhibits Hedgehog (Hh) signaling with similar or lower toxicity (IC₅₀ = 20 nM in Shh-LIGHT2 assay; 50 nM in p2^Ptch-/-cells; 500 nM in SmoA1-LIGHT cells). Binds to SmoA1 and promotes its exit from the endoplasmic reticulum. Suppresses both ShhNp-induced pathway activity and SmoA1-induced reporter activity. Reported to cause regression of murine tumor allografts in vivo and induce rapid cell death in human medulloblastoma. Shown to sensitize human glioma cells to TRAIL-induced apoptosis. The solid form of this compound (Cat. No. 239803) is also available.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
SmoA1

包装

Packaged under inert gas

警告

Toxicity: Irritant (B)

物理的形状

A 1 mM (50 µg/72 µL) solution of Cyclopamine-KAAD (Cat. No. 239804) in DMSO.

再構成

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

その他情報

Siegelin, M.D. et al. 2009. Neurobiol. Dis.34, 259.
Watkins, D.N., et al. 2003. Nature422, 313.
Berman, D.M., et al. 2002. Science297, 1559.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Chen, J.K., et al. 2002. Genes Dev.16, 2743.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.
Taipale, J., et al. 2000. Nature406, 1005.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): 188.6 °F - closed cup - (refers to pure substance)
• 引火点(℃): 87 °C - closed cup - (refers to pure substance)