16-204

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^®, biotin conjugate

clone 4G10®, Upstate^®, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: biotin conjugate
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 4G10®, monoclonal
• 交差性（ホモロジーによる予測）: all
• メーカー／製品名: Upstate^®
• テクニック: western blot: suitable
• アイソタイプ: IgG2bκ
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PID1(55022)

詳細

詳細

Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.

特異性

Tyrosine-phosphorylated proteins from all species.

免疫原

Phosphotyramine-KLH

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
一般的な翻訳後修飾

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10, biotin conjugate validated for use in WB.

品質

Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

ターゲットの説明

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

物理的形状

Protein G Purified

Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.

保管および安定性

Stable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!

アナリシスノート

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2