1.25184

Fractogel^® EMD SO₃⁻ (S) Resin

RoboColumn Fractogel^® SO3 (S), volume 0.6 mL, prepacked column

• 別名: RoboCol Fractogel^® SO3 (S), 0.6ml
• UNSPSCコード: 41115711

特性

• ligand: (Sulfoisobutyl)
• 品質水準: 100
• 物質: polypropylene housing
• 製品種目: Fractogel^®
• 形状: prepacked column
• パラメーター: ≤8 bar pressure; ~80 cm/hr max. flow rate
• column I.D. × L: 5 mm × 30 mm
• 容量: 0.6 mL
• マトリックス活性基: Methacrylate
• 平均粒径: 20-40 μm
• キャパシティ: ~150 mL, resin binding capacity
• アプリケーション: recombinant protein; vaccine development
• 保管温度: no temp limit

詳細

詳細

5 x 30 mm

特徴および利点

RoboColumn Fractogel^® SO3 (S), 0.6ml enables:

• Efficient separation between monomer, dimer, and high molecular weight aggregates
• High binding capacity
• High resolution
• Efficient polishing step
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

包装

Plastic bag(s)

法的情報

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany