05-807

Anti-phospho-CREB (Ser133) Antibody, clone 634-2

clone 634-2, Upstate^®, from mouse

• 別名: active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 634-2, monoclonal
• 化学種の反応性: rat, mouse
• メーカー／製品名: Upstate^®
• テクニック: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_134442
• UniProtアクセッション番号: P16220
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (pSer133)
• 遺伝子情報: human ... CREB1(1385)

詳細

詳細

CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

特異性

Broad species cross-reactivity expected, based on sequence homology.

Phospho-CREB (Ser133).

免疫原

A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.

Epitope: Ser133

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Beadlyte Specificity Assay:
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence

Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.

Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.

品質

Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.

Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).

ターゲットの説明

43 kDa

物理的形状

Protein G Purified

Format: Purified

Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.

保管および安定性

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

アナリシスノート

Control
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1