おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
7A6, monoclonal
化学種の反応性
mouse, Saccharomyces cerevisiae, human
メーカー／製品名
Upstate®
テクニック
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... PPP2CA(5515)
特異性
Recognizes the catalytic subunit of PP2A.
免疫原
peptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit
アプリケーション
Research Category
細胞シグナル伝達
ニューロサイエンス
細胞シグナル伝達
ニューロサイエンス
Research Sub Category
キナーゼ及びホスファターゼ
神経変性疾患
キナーゼ及びホスファターゼ
神経変性疾患
Anti-PP2A Antibody, C subunit, clone 7A6 detects level of PP2A & has been published & validated for use in IP & WB.
品質
routinely evaluated by immunoblot on RIPA lysates from A431 cells
ターゲットの説明
36 kDa
物理的形状
Unpurified
Cell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative.
保管および安定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-545:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Loss of protein phosphatase 2A expression correlates with phosphorylation of DP-1 and reversal of dysplasia through differentiation in a conditional mouse model of cancer progression.
Maddalena T Tilli, Shawnte L Hudgins, M Silvina Frech, Ewa D Halama, Jean-Pierre Renou et al.
Cancer Research null
Chang Y Guo et al.
The Journal of biological chemistry, 277(7), 4839-4844 (2001-11-28)
Ionizing radiation (IR) is known to activate multiple cell cycle checkpoints that are thought to enhance the ability of cells to respond to DNA damage. Protein phosphatase 2A (PP2A) has been implicated in IR-induced activation of checkpoints; therefore, Jurkat cells
Regulation of L-type calcium channel and delayed rectifier potassium channel activity by p21-activated kinase-1 in guinea pig sinoatrial node pacemaker cells.
Ke, Y; Lei, M; Collins, TP; Rakovic, S; Mattick, PA; Yamasaki, M; Brodie, MS; Terrar, DA; Solaro, RJ
Circulation Research null
N Begum et al.
The Journal of biological chemistry, 271(49), 31166-31171 (1996-12-06)
In this study, we examined the mechanism of recently reported inactivation of protein phosphatase-2A (PP-2A) by insulin (Srinivasan, M., and Begum, N. (1994) J. Biol. Chem. 269, 12514-12520) and its counter-regulation by cAMP agonists. Exposure of L6 myotubes to insulin
A Fischer et al.
Toxicology and applied pharmacology, 245(1), 9-20 (2010-02-23)
Cellular uptake of microcystins (MCs), a family of cyclic cyanobacterial heptapeptide toxins, occurs via specific organic anion transporting polypeptides (OATPs), where MCs inhibit serine/threonine-specific protein phosphatase (PP). Despite comparable PP-inhibitory capacity, MCs differ greatly in their acute toxicity, thus raising
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