800814P

14:0 DG

1,2-dimyristoyl-sn-glycerol, powder

• 別名: 1,2-ditetradecanoyl-sn-glycerol; DG(14:0/14:0/0:0)
• 実験式（ヒル表記法）: C₃₁H₆₀O₅
• CAS番号: 60562-16-5
• 分子量: 512.81
• UNSPSCコード: 12352211
• NACRES: NA.25

特性

• アッセイ: >99% (TLC)
• 形状: powder
• 包装: pkg of 1 × 10 mg (800814P-10mg); pkg of 1 × 25 mg (800814P-25mg)
• メーカー／製品名: Avanti Research^™ - A Croda Brand 800814P
• 脂質タイプ: neutral glycerides; neutral lipids
• 輸送温度: dry ice
• 保管温度: −20°C
• InChI: 1S/C31H60O5/c1-3-5-7-9-11-13-15-17-19-21-23-25-30(33)35-28-29(27-32)36-31(34)26-24-22-20-18-16-14-12-10-8-6-4-2/h29,32H,3-28H2,1-2H3/t29-/m0/s1
• InChI Key: JFBCSFJKETUREV-LJAQVGFWSA-N

詳細

詳細

1,2-dimyristoyl-sn-glycerol (14:0 DG) with small polar headgroup and two fatty acid, is an E coli membrane lipid.

In biochemical signaling, diacylglycerol (DAG) functions as a second messenger signaling lipid, and is a product of the hydrolysis of the phospholipid PIP2 (phosphatidylinositolbisphosphate) by the enzyme phospholipase C (PLC) (a membrane-bound enzyme) that, through the same reaction, produces inositol trisphosphate (IP3). Although inositol trisphosphate (IP3) diffuses into the cytosol, DAG remains within the plasma membrane due to its hydrophobic properties. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, whereas DAG is a physiological activator of protein kinase C (PKC). The production of DAG in the membrane facilitates translocation of PKC from the cytosol to the plasma membrane.
Diacylglycerol mimicks the effects of the tumor-promoting compounds phorbol esters.

アプリケーション

14:0 DG may be used:

• in the reconstitution of dry lipids for thin layer chromatography
• in lipid nanoparticles for RNA delivery studies
• as a standard in gas chromatography–mass spectrometry (GC-MS) analysis for the quantification of lipid A diacylglycerols

生物化学的／生理学的作用

Diacylglycerol (DAG) displays a flip-flop motion. This movement of DAG is inhibited by membrane protein integrase (mini-MPIase-3).It also forms nonlamellar phase.

包装

5 mL Amber Glass Screw Cap Vial (800814P-10mg)

5 mL Amber Glass Screw Cap Vial (800814P-25mg)

保管および安定性

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

その他情報

Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

法的情報

Avanti Research is a trademark of Avanti Polar Lipids, LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable