T4184

Thrombopoietin from mouse

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

• Synonym(s): TPO
• CAS Number: 9014-42-0
• MDL number: MFCD01095336
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in NSO cells
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 2.1 ng/mL EC₅₀
• mol wt: ~75 kDa by SDS-PAGE (as a result of glycosylation); predicted mol wt 35 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P40226
• storage temp.: −20°C
• Gene Information: mouse ... Thpo(21832)

Description

Application

Thrombopoietin from mouse can be used in a genetic screening test for examining the role of somatic activating mutations in EPOR, MPL, or GCSFR genes in activating myeloproliferative disorders (MPD) in JAK2V617F-negative MPD patients.[1]

Biochem/physiol Actions

TPO is a ligand for the c-mpl proto-oncogene (Mpl-L), a megakaryocyte colony stimulating factor (Meg-CSF), and a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates a 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved, showing approximately 70% sequence homology among various mammals.

Thrombopoeitin (TPO), also known as the ligand for the c-mpl proto-oncogene (Mpl-L) and as megakaryocyte colony stimulating factor (Meg-CSF), is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. Produced mainly by hepatocytes but also by kidney cells, TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved glycoprotein, showing approximately 70% sequence homology among various mammals. Human TPO contains 332 amino acids (mouse 335) including two internal disulfide bonds and 6 N-linked glycosylation sites (mouse 7). The N-terminal 153 amino acid region of TPO shows structural and amino acid sequence homology to erythropoietin and a pair of arginines (potential cleavage site) separating it from the highly glycosylated carboxyterminal domain.

Thrombopoietin from mouse is an amino acid peptide that plays a pivotal role in megakaryocyte proliferation and differentiation.[2] It also serves as a growth factor for liver endothelial cells in mouse.[3]

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin

Analysis Note

The biological activity was measured in a cell proliferation assay using MO7e cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves