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Key Documents

Safety Information

SRE0055

Sigma-Aldrich

Protease Inhibitor Cocktail Powder

Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.

Synonym(s):

Protease Inhibitor Cocktail powder

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

bovine lung
yeast

form

lyophilized powder

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, and metallo-proteases.

Application

Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.

Components

1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRE0055-1BTL:
SRE0055-1BO:
SRE0055-VAR:
SRE0055-1BO-PW:
SRE0055-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
105M4022V
11151003
11151002
11151001
105M4024V

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