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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Synonym(s):

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
form:
DMSO solution
storage temp.:
−20°C

Product Name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

200

packaging

pkg of 1 ml, 5 ml ×

shipped in

wet ice

storage temp.

−20°C

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction
  • to lyse purified CD4+ T cells
  • to prepare protein extracts from placental tissues and cells

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

JAN Code

PPC1010-1ML-PW:
PPC1010-BULK:
PPC1010-1ML:
PPC1010-5ML:
PPC1010-5ML-PW:
PPC1010-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
0000201980
0000359849
0000359848
0000359849
0000359848

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