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S8629

Sigma-Aldrich

Sulfatase from Patella vulgata (keyhole limpet)

Type V, essentially salt-free, lyophilized powder, ≥5 units/mg solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥5 units/mg solid

foreign activity

β-Glucuronidase ≤2 unit/mg solid

storage temp.

−20°C

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Application

Sulfatase from Patella vulgata has been used in a study to validate a method for the quantitation of quercetin from human plasma. Sulfatase from Patella vulgata has also been used in a study to compare properties of human lung SRS-A to distinct fractions from ionophore-induced rat mononuclear cell SRS.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S8629-100UN:
S8629-1KU:
S8629-250UN:
S8629-5KU:
S8629-VAR:
S8629-BULK:
S8629-500UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D J Smith et al.
Journal of animal science, 80(5), 1240-1249 (2002-05-22)
Ractopamine HCl is a beta-adrenergic leanness-enhancing agent recently approved for use in swine. Depletion of ractopamine in tissues, and elimination of ractopamine and its metabolites in urine, is of interest for the detection of off-label use. The objectives of this
I Erlund et al.
Journal of chromatography. B, Biomedical sciences and applications, 727(1-2), 179-189 (1999-06-09)
A validated method for the quantitation of trace levels of quercetin from human plasma to be used in pharmacokinetic and biomarker studies is presented. Quercetin conjugates were hydrolysed enzymatically, plasma proteins were removed using a Bond Elut C18 extraction column
Slow reacting substances: comparison of some properties of human lung SRS-A and two distinct fractions from ionophore-induced rat mononuclear cell SRS.
M K Bach et al.
Journal of immunology (Baltimore, Md. : 1950), 122(1), 160-165 (1979-01-01)
Bradley J Stevenson et al.
Drug testing and analysis, 7(10), 903-911 (2015-03-06)
The hydrolysis of sulfate ester conjugates is frequently required prior to analysis for a range of analytical techniques including gas chromatography-mass spectrometry (GC-MS). Sulfate hydrolysis may be achieved with commercial crude arylsulfatase enzyme preparations such as that derived from Helix
S Saha et al.
Pharmacological research, 65(6), 592-602 (2012-03-01)
Epicatechin is a widely consumed dietary flavonoid and there is substantial evidence that it contributes to the health benefits reported for flavanol-rich cocoa products including dark chocolate. Numerous reports have described the appearance of epicatechin and epicatechin phase-2 conjugates (sulfates
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