Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

SAE0213

Sigma-Aldrich

Arylsulfatase, Enhanced, Recombinant

From P. aeruginosa for Steroid Sulfate Hydrolysis

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
EC 3.1.6.1
UNSPSC Code:
12352204
NACRES:
NA.77

Quality Level

200

form

liquid

specific activity

≥100 units/mg protein

mol wt

60 kDa

shipped in

dry ice

storage temp.

-10 to -25°C

General description

Enhanced arylsulfatase is a recombinantly expressed form of the arylsulfatase found in Pseudomonas arigenosa, with introduced mutations for more efficient catalysis of steroidal sulfate esters. It is useful for sample preparation of urine metabolites (sulfated drug and endogenous conjugates) before analysis by HPLC and/or Mass Spec. It will hydrolyze sulfoconjugates to improve retention time and consolidate parent analyte peaks on HPLC.

Application

Useful for endogenous and xenobiotic and drug metabolite analysis.

Biochem/physiol Actions

(Aryl)sulfatases are used by the body for the catabolism of sulfated compounds, which play a crucial role in a number of physiological processes. Some examples include sulfated glucosaminoglycans like heparin, hyaluronan and chondroitin. This particular arylsulfatase is derived from microbial source and is used as a tool for processing sulfated metabolites, rather than for its physiological purpose.

Physical form

Supplied as buffered solution containing 20 mM Hepes pH7.8, 150 mM NaCl, 10% v/v Glycerol and 0.5 mM CaCl2.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0213-5KU:
SAE0213-VAR:
SAE0213-50KU:
SAE0213-BULK:
SAE0213-10KU:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service