PP0201

Glycoprofile^™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

• NACRES: NA.26
• UNSPSC Code: 12352202

Properties

• Quality Segment: 200
• storage temp.: 2-8°C

Description

General description

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Application

GlycoProfile^™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.

GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.

Glycoprofile^™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• 10× Reaction Buffer 1 mL/vial

Kit Components Also Available Separately

• P7367

  PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U

  SDS
• R7884

  Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg

  SDS
• O9882

  Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg

  SDS
• M3148

  2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL

  SDS

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Codes: H301 + H331,H310,H315,H317,H318,H334,H361f,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Target Organs: Liver,Heart
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3