Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

PP0200

Sigma-Aldrich

GlycoProfile™ I, In-Gel Deglycosylation Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

EC Number:
232-615-7
UNSPSC Code:
12352200
NACRES:
NA.32

shelf life

≥1 yr at 2‑8 °C

Quality Level

200

storage temp.

2-8°C

Application

The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Principle

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantity

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDS
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
  • Trypsin Solubilization Reagent
  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomicsSDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

35.6 °F

Flash Point(C)

2.0 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

Please refer to KIT Component information

FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

O-Linked Glycan Strategies

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service