MAK535

Glutathione Reductase Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• detection method: colorimetric
• relevant disease(s): neurological disorders; cardiovascular diseases; hematological disorder; cancer
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Glutathione reductase (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress

Features and Benefits

• Linear detection range: 0.4 to 50 U/L
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for glutathione reductase activity determination in biological samples (e.g. plasma, serum, tissue, and culture media).

Principle

The Glutathione Reductase Kit is designed to accurately measure GR activity in biological samples with a protocol that utilizes Ellman′s method, in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3