MAK314

Sialic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Sialic Acid (NANA) Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• relevant disease(s): cancer; cardiovascular diseases; hematological disorder
• storage temp.: −20°C

Description

General description

Sialic Acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). They are associated with carbohydrates and are localized on cell membranes. Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer.

Application

• Evaluation of serum total sialic acid in moderate COVID-19 patients with and without gastrointestinal tract manifestations.: The study measures serum sialic acid levels in COVID-19 patients to understand the disease′s impact on the gastrointestinal tract (Haroun et al., 2022).

Suitability

Suitable for the quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid in serum, plasma, saliva, milk etc.

Principle

This sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid. This reacts with thiobarbituric acid to form a pink colored product. The colorimetric (549nm)/fluorimetric (λex = 555nm/λem = 585nm) product is directly proportional to sialic acid concentration in the sample.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H290,H314,H335,H411
• Precautionary Statements: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable