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MAK145

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (red)

Synonym(s):

Intracellular ROS Fluorescence Assay

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¥52,020

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1 KIT
¥52,020

About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

¥52,020

Check Cart for Availability
Request a Bulk Order

usage

sufficient for 200 fluorometric tests (red)

detection method

fluorometric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

Related Categories

General description

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense.[1] Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[2]

Application

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.[3][4][5][6]

Suitability

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.

Principle

The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

キットコンポーネントの情報を参照してください

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Certificates of Analysis (COA)

Lot/Batch Number
3511823
3511823
3321242
318090
304001

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Sean A Misek et al.
Frontiers in oncology, 12, 766794-766794 (2022-04-22)
Single agent and combination therapy with BRAFV600E/K and MEK inhibitors have remarkable efficacy against melanoma tumors with activating BRAF mutations, but in most cases BRAF inhibitor (BRAFi) resistance eventually develops. One resistance mechanism is reactivation of the ERK pathway. However
Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 null
Yara E Elakkad et al.
Breast cancer (Dove Medical Press), 13, 675-689 (2021-12-23)
Female breast cancer is the most prevalent cancer worldwide. Emerging evidence shows that simvastatin (SIM) has promising anticancer activities. However, the underlying mechanisms are not fully elucidated. Increasing reports imply statins can modulate ferroptosis through disrupting reactive oxygen species (ROS)
Jianyong Yu et al.
Experimental and therapeutic medicine, 22(3), 946-946 (2021-07-27)
Salinomycin is a polyether antiprotozoal antibiotic that is widely used as an animal food additive. Some antifungal, antiparasitic, antiviral and anti-inflammatory activities have been reported for salinomycin. Recently, the anti-cancer effect of salinomycin has been demonstrated in breast cancer; however
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in Endocrinology, 2, 53-53 (2014)
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the spectral range of the fluorophore?

    1 answer

    1. The fluorophore has excitation at 520 nm and emission at 605 nm which is important for determining fluorescence intensity.

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