MAK095

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; neurological disorders
• storage temp.: −20°C

Description

General description

Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.

Application

Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.

Suitability

The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup