MAK085

Lipid Peroxidation (MDA) Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): MDA Assay, TBARS Assay, Thiobarbituric Acid Reactive Substances Assay
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• drug control: regulated under CDSA - not available from Sigma-Aldrich Canada
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases
• storage temp.: −20°C

Description

General description

Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes, and Alzheimer′s.

Application

Lipid peroxidation (MDA) assay kit has been used to determine the levels of malondialdehyde (MDA).

Suitability

Suitable for the measurement of malondialdehyde (MDA) in a variety of samples including tissue, cells and plasma

Principle

In this kit, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a colorimetric (532 nm)/fluorometric (λ_ex = 532/λ_em = 553 nm) product, proportional to the MDA present.

Safety Information

• Pictograms: GHS02,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H225,H302,H314,H411
• Precautionary Statements: P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1C
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 57.2 °F - closed cup
• Flash Point(C): 14 °C - closed cup