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L3382

Sigma-Aldrich

Lectin from Helix pomatia

lyophilized powder, salt, free

Synonym(s):

Helix pomatia agglutinin, HPA

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1 MG
¥31,500
5 MG
¥112,000

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

¥31,500

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New, lower price on this item!

form

lyophilized powder

potency

<4 μg per mL agglutination activity

impurities

salt, free

storage temp.

−20°C

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General description

Lectin from Helix pomatia is a hexameric glycoprotein[1] with a molecular mass of 79 kDa.[2] It issecreted by the albumen gland of the roman snail.[2] Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).[1]

Application

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium[3] and used in lectin-agglutination tests.[4]

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid.[2] They exhibit antibacterial activities and immunity in snail.[2] Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens.[1] HPA has also emerged as a marker of altered Oglycosylation in cancer.[5]

Preparation Note

Affinity purified by a modification of the method of Hammarstrom and Kabat.[6]

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L3382-5MG:
L3382-.5MG:
L3382-BULK:
L3382-VAR:
L3382-10MG:
L3382-1MG:

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Certificates of Analysis (COA)

Lot/Batch Number
0000387198
0000356695
0000356696
0000358287
0000358286

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Katie Barr et al.
Transfusion, 55(1), 129-136 (2014-07-22)
The FORS blood group system (originally recognized as the Apae phenotype) was discovered by sporadic activity against polyclonal anti-A reagents and activity against the lectin Helix pomatia. The extent of monoclonal anti-A reagent activity against the FORS1 antigen is serologically
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients
Reily C, et al.
Biotechniques, 65(2), 71-77 (2018)
Characterization of bovine and human group B streptococci isolated in Turkey
Ekin IH and Gurturk K
Journal of Medical Microbiology, 55(5), 517-521 (2006)
Binding of aberrant glycoproteins recognizable by Helix pomatia agglutinin in adrenal cancers
Parameswaran R, et al.
BJS open, 2(5), 353-359 (2018)
Biochemical and structural analysis of helix pomatia agglutinin a hexameric lectin with a novel fold
Sanchez JF, et al.
The Journal of biological chemistry, 281(29), 20171-20180 (2006)
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