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Safety Information

L1412

Sigma-Aldrich

Lysing Enzymes from Trichoderma harzianum

lyophilized powder

Synonym(s):

Glucanex®

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About This Item

UNSPSC Code:
12352204

form

lyophilized powder

specific activity

≥10 units/g

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Application

Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
  • for protoplast isolation[1]
  • for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation[2]
  • for the lysis of Aspergillus niger vegetative cells[3]

Biochem/physiol Actions

Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.[4]

Quality

Contains β-glucanase, cellulase, protease, and chitinase activities

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).

Legal Information

A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L1412-VAR:
L1412-BULK:
L1412-25G:
L1412-10G:
L1412-5G:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBJ0553V
SLBG2959V
SLBB7969V
080M1447V
080M1447

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