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D5292

Sigma-Aldrich

DNA Ladder, Supercoiled

for supercoiled DNA electrophoresis

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About This Item

UNSPSC Code:
12352200

grade

for molecular biology

form

liquid

usage

250 uses (25 μg size)
50 uses (10 μg size)

suitability

suitable for electrophoresis (DNA)

shipped in

dry ice

storage temp.

−20°C

General description

Sigma′s DNA Ladder (Supercoiled) contains 11 supercoiled DNA fragments from 2,067 – 16,210 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Components

Sigma′s DNA Ladder (Supercoiled) is provided in a solution of 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.

Other Notes

Contains 11 supercoiled fragments, 2,067–16,210 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D5292-25UG:
D5292-BULK:
D5292-10UG:
D5292-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBD1269
041M1454
080M1675
099K0981
018K1083

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Michael J Molloy et al.
Nucleic acids research, 32(16), e129-e129 (2004-09-11)
Within the biopharmaceutical industry, recombinant plasmid DNA is used both as a raw material (e.g. in lentiviral and AAV vector production) as well as an active ingredient (e.g. in DNA vaccines). Consequently, many analytical laboratories are routinely involved with plasmid

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